Bee Propolis / Propolisin Herpes etkileri Bilimsel çalışmalar.

Propolis & Herpes

1: Isr Med Assoc J. 2002 Nov;4(11 Suppl):923-7.

Anti-herpes simplex virus effect of an aqueous extract of propolis.

Huleihel M, Isanu V.

Institute for Applied Biosciences, Ben-Gurion University of the Negev, Beer Sheva, Israel. mahmoudh@bgumail.bgu.ac.il

BACKGROUND: Propolis, a natural product from beehives, comprises a complex of chemicals, the most important group being flavinoids, which play a role in antiviral protection. OBJECTIVES: To test the inhibitory effect of propolis extract against herpes simplex viruses in vitro and in vivo. METHODS: In vitro: propolis was added to Vero cells at various times and concentrations before, at or after infection with HSV-1. In vivo: the effect of propolis was tested in newborn rats infected s.c. or i.p. and on rabbit come as infected with HSV-1. RESULTS: In vitro: 0.5% propolis extract caused 50% inhibition of HSV infection. There was indirect evidence for a strong interaction between the propolis extract and the surface of the Vero cells, but there was no direct interaction with HSV-1 particles. Administration of propolis before or at the time of infection yielded the most significant inhibitory effect, but even when 10% propolis extract was added 2 hours post-infection it gave 80-85% protection. In vivo: as little as 5% propolis prevented the appearance and development of symptoms of local and i.p. HSV-1 infection in rats and of corneal HSV-1 infection in rabbits. There were nocytotoxic effects at a concentration of 10% in vitro or 20% in vivo. CONCLUSIONS: The potent antiviral activity of propolis against HSV-1 infection in vitro and In vivo is probably due to prevention of virus absorption into the host cells and/or inhibition of an internal step(s) during the viral replication cycle.

PMID: 12455181 [PubMed - indexed for MEDLINE]

2: Phytomedicine. 2000 Mar;7(1):1-6.

A comparative multi-centre study of the efficacy of propolis, acyclovir and placebo in the treatment of genital herpes (HSV).

Vynograd N, Vynograd I, Sosnowski Z.

Institute of Epidemiology, Lvov State Medical University, Ukraine.

Ninety men and women with recurrent genital HSV type 2 participated in a randomized, single-blind, masked investigator, controlled multi-centre study comparing the efficacy of ointment of Canadianpropolis containing natural flavonoids with ointments of acyclovir and placebo (vehicle) on healing ability and capacity to remedy symptoms. Thirty individuals were randomized to each group. Treatment was intended to start in the blister phase. All participants had HSV type 2 isolated, confirmed by serum immunoglobulin levels. The participants were examined on the 3rd, 7th and 10th days of treatment by gynaecologists, dermatovenerologists or urologists at seven different medical centres. Apart from clinical symptoms the number and size of the herpetic lesions were noted. At each examination the lesions were classified into four stages: vesicular, ulcerated, crusted and healed. The study ointments were applied to affected areas four times daily. In women with vaginal or cervical lesions a tampon with the appropriate ointment was inserted four times daily for 10 days. Endpoint variables were healing time and time until loss of symptoms. RESULTS: On Day 10, 24 out of 30 individuals in the propolis group had healed. In the acyclovir group 14 out of 30 and in the placebo group 12 out of 30 had healed. (p = 0.0015). The healing process appeared to be faster in thepropolis group. In the propolis group 15 individuals had crusted lesions on Day 3 compared to 8 individuals in the acyclovir group and none in the placebo group (p = 0.0006). On Day 7, 10 participants in the propolis group, 4 in the acyclovir group and 3 in the placebo group had healed. At the initial examination all patients had local symptoms and 28% general symptoms. At Day 3, 3 patients in the propolis group had local symptoms compared to 8 and 9 in the acyclovir and placebo groups respectively. Of the women, 66% had vaginal superinfections of microbial pathogens at the initial examination. In the acyclovir and placebo groups no change in the vaginal flora was found following treatment whereas in the propolis group the incidence of superinfection was reduced by 55%. (p = 0.10 n.s.). CONCLUSION: An ointment containing flavonoids appeared to be more effective than both acyclovir and placebo ointments in healing genital herpetic lesions, and in reducing local symptoms.

PMID: 10782483 [PubMed - indexed for MEDLINE]

3: Voen Med Zh. 1995 Dec;(12):36-9, 80.

[The use of ocular drug films of propolis in the sequelae of ophthalmic herpes]

[Article in Russian]

Maichuk IuF, Orlovskaia LE, Andreev VP.

There was studied the therapeutic efficiency of ocular medical propolis films (OMF) in 35 patients with postherpetic trophic keratitis and in 20 with postherpetic nebula. OMF were applied behind the lower eyelid at bedtime during 10-15 days. All the patients endured the propolis films well. OMF accelerated the cornea epithelization. Epitheliopathy and micropoint edema of cornea epithelium rapidly disappeared. Time of patients recovery reduced nearly twice (P,001) in comparison with the control group--from 14.1 to 7.6 days. On the average their visual acuity increased in two times--from 0.12 to 0.27 (P > 0.001).

PMID: 8779164 [PubMed - indexed for MEDLINE]

4: J Nat Prod. 1994 May;57(5):644-7.

Comparison of the anti-herpes simplex virus activities of propolis and 3-methyl-but-2-enyl caffeate.

Amoros M, Lurton E, Boustie J, Girre L, Sauvager F, Cormier M.

Laboratoire de Pharmacognosie et Mycologie.

The in vitro activity against herpes simplex virus type 1 of 3-methyl-but-2-enyl caffeate isolated from poplar buds or prepared by synthesis was investigated. Under conditions of one or multiple multiplication cycles, this compound, which is a minor constituent of propolis, was found to reduce the viral titer by 3 log10, and viral DNA synthesis by 32-fold.

PMID: 8064297 [PubMed - indexed for MEDLINE]

5: J Nat Prod. 1992 Dec;55(12):1732-40.

Synergistic effect of flavones and flavonols against herpes simplex virus type 1 in cell culture. Comparison with the antiviral activity of propolis.

Amoros M, Simoes CM, Girre L, Sauvager F, Cormier M.

Laboratoire de Pharmacognosie et Mycologie, Faculte de Pharmacie, Universite de Rennes I, France.

The in vitro activity against herpes simplex virus type 1 of the major flavonoids identified in propolis was investigated. Flavonols were found to be more active than flavones, the order of importance being galangin, kaempferol, and quercetin. The efficacy against HSV-1 of binary flavone-flavonol combinations has been also investigated. The synergy demonstrated by all combinations could explain why propolis is more active than its individual compounds.

PMID: 1338212 [PubMed - indexed for MEDLINE]

6: Microbiologica. 1990 Jul;13(3):207-13.

Effects of propolis flavonoids on virus infectivity and replication.

Debiaggi M, Tateo F, Pagani L, Luini M, Romero E.

Istituto di Microbiologia, Universita degli Studi di Pavia, Italy.

The effect of five propolis flavonoids on the infectivity and replication of some herpesvirus, adenovirus, coronavirus and rotavirus strains has been studied. Experiments were performed in vitro in cell cultures using the viral plaque reduction technique. The cytotoxicity of flavonoids, including chrysine, kaempferol, acacetin, galangin and quercetin, was evaluated on uninfected monolayers to determine their effect on cell growth and viability. Chrysine and kaempferol caused a concentration-dependent reduction of intracellular replication of herpes-virus strains when monolayers were infected and subsequently cultured in a drug-containing medium. However, virus infectivity was not significantly affected. Acacetin and galangin had no effect on either the infectivity or replication of any of the viruses studied. Quercetin reduced infectivity and intracellular replication, but only at the highest concentrations tested.

PMID: 2125682 [PubMed - indexed for MEDLINE]

7: Virologie. 1988 Jan-Mar;39(1):21-4.

[Treatment of cutaneous herpes and herpes zoster with Nivcrisol-D]

Giurcaneanu F, Crisan I, Esanu V, Cioca V, Cajal N.

Hopital Colentina, Service de Dermatologie, Bucarest, Roumanie.

The results obtained at the Dermatological service of the Colentina Hospital show that the product NIVCRISOL-D, containing propolis, has a significant therapeutical effect against recurrent herpes and zona zoster.

PMID: 3376426 [PubMed - indexed for MEDLINE]

8: Virologie. 1984 Oct-Dec;35(4):281-93.

Research in the field of antiviral chemotherapy performed in the "Stefan S. Nicolau" Institute of Virology.

Esanu V.

A brief review is made of the research in the field of antiviral chemotherapy performed in the "Stefan S. Nicolau" Institute of Virology during the 35 years since its foundation. The investigations have mainly focused on influenza and herpes virus, but the chemotherapy of other viral infections (mumps, vaccinia, Coxsackie, etc.) has also been approached. Most of the chemotherapy agents assayed have been represented by natural preparations: immunoglobulins, interferon, hormones, vitamins, plant extracts (garlic, horse radish), bee products (propolis, royal jelly); attempts have also been made with numerous synthetic compounds. Stress is laid on the preparations already tested with a view to application in human clinic, and the prospects of chemotherapy research in the Institute of Virologyare discussed.

PMID: 6097022 [PubMed - indexed for MEDLINE]

9: Virologie. 1981 Jan-Mar;32(1):57-77.

Recent advances in the chemotherapy of herpes virus infections.

Esanu V.

The main categories of antiherpes agents presently used in chemotherapy area reviewed according to the phase of virus replication affected : 1) virus adsorption (adamantane, nonionic surfactants) ; 2) eclipse (interferon) ; 3) virion maturation (nucleoside and nucleotide analogues and phosphonic acid derivatives). Mention is also made of other compounds--different synthetic organic derivatives, photodynamic dyes, metal ions, boric acid, hormones, antibiotics, other natural products (extracts from marine algae, propolis, garlic)--with promising antiviral properties. The difficulties and prospects of viral chemotherapy research are briefly discussed.

PMID: 6191437 [PubMed - indexed for MEDLINE]

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Pterostilben:Antibakteriyel etki (Walker ve Crane ,1987),Sakuraretin:Antibakteriyel etki (Walker ve Crane,1987)